PoplarV1, Main, Exploration, bibRecord, 003A89

Protein extraction from woody plants.

Identifieur interne : 003A89 ( Main/Exploration ); précédent : 003A88; suivant : 003A90

Protein extraction from woody plants.

Auteurs : Christophe Plomion [France] ; Céline Lalanne

Source :

Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2007.

RBID : pubmed:17093300

Descripteurs français

KwdFr :
- Feuilles de plante (composition chimique), Phloème (composition chimique), Pinus (composition chimique), Pollen (composition chimique), Populus (composition chimique), Protéines végétales (composition chimique), Protéines végétales (isolement et purification), Protéomique (méthodes), Précipitation chimique (MeSH), Quercus (composition chimique), Racines de plante (composition chimique), Solubilité (MeSH), Xylème (composition chimique).
MESH :
- composition chimique : Feuilles de plante, Phloème, Pinus, Pollen, Populus, Protéines végétales, Quercus, Racines de plante, Xylème.
- isolement et purification : Protéines végétales.
- méthodes : Protéomique.
- Précipitation chimique, Solubilité.

English descriptors

KwdEn :
- Chemical Precipitation (MeSH), Phloem (chemistry), Pinus (chemistry), Plant Leaves (chemistry), Plant Proteins (chemistry), Plant Proteins (isolation & purification), Plant Roots (chemistry), Pollen (chemistry), Populus (chemistry), Proteomics (methods), Quercus (chemistry), Solubility (MeSH), Xylem (chemistry).
MESH :
- chemical , chemistry : Plant Proteins.
- chemistry : Phloem, Pinus, Plant Leaves, Plant Roots, Pollen, Populus, Quercus, Xylem.
- chemical , isolation & purification : Plant Proteins.
- methods : Proteomics.
- Chemical Precipitation, Solubility.

Abstract

In this chapter we present a protocol for total protein extraction optimized for wood-forming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php.

DOI: 10.1385/1-59745-227-0:37
PubMed: 17093300

Affiliations:

France

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 003C84
to stream Main, to step Curation: 003C84

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protein extraction from woody plants.</title>
<author><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name>
<affiliation wicri:level="1"><nlm:affiliation>NRA, UMR BIOGECO, Equipe de Génétique, Cestas, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>NRA, UMR BIOGECO, Equipe de Génétique, Cestas</wicri:regionArea>
<wicri:noRegion>Cestas</wicri:noRegion>
<wicri:noRegion>Cestas</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lalanne, Celine" sort="Lalanne, Celine" uniqKey="Lalanne C" first="Céline" last="Lalanne">Céline Lalanne</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2007">2007</date>
<idno type="RBID">pubmed:17093300</idno>
<idno type="pmid">17093300</idno>
<idno type="doi">10.1385/1-59745-227-0:37</idno>
<idno type="wicri:Area/Main/Corpus">003C84</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003C84</idno>
<idno type="wicri:Area/Main/Curation">003C84</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003C84</idno>
<idno type="wicri:Area/Main/Exploration">003C84</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Protein extraction from woody plants.</title>
<author><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name>
<affiliation wicri:level="1"><nlm:affiliation>NRA, UMR BIOGECO, Equipe de Génétique, Cestas, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>NRA, UMR BIOGECO, Equipe de Génétique, Cestas</wicri:regionArea>
<wicri:noRegion>Cestas</wicri:noRegion>
<wicri:noRegion>Cestas</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lalanne, Celine" sort="Lalanne, Celine" uniqKey="Lalanne C" first="Céline" last="Lalanne">Céline Lalanne</name>
</author>
</analytic>
<series><title level="j">Methods in molecular biology (Clifton, N.J.)</title>
<idno type="ISSN">1064-3745</idno>
<imprint><date when="2007" type="published">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chemical Precipitation (MeSH)</term>
<term>Phloem (chemistry)</term>
<term>Pinus (chemistry)</term>
<term>Plant Leaves (chemistry)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (isolation & purification)</term>
<term>Plant Roots (chemistry)</term>
<term>Pollen (chemistry)</term>
<term>Populus (chemistry)</term>
<term>Proteomics (methods)</term>
<term>Quercus (chemistry)</term>
<term>Solubility (MeSH)</term>
<term>Xylem (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (composition chimique)</term>
<term>Phloème (composition chimique)</term>
<term>Pinus (composition chimique)</term>
<term>Pollen (composition chimique)</term>
<term>Populus (composition chimique)</term>
<term>Protéines végétales (composition chimique)</term>
<term>Protéines végétales (isolement et purification)</term>
<term>Protéomique (méthodes)</term>
<term>Précipitation chimique (MeSH)</term>
<term>Quercus (composition chimique)</term>
<term>Racines de plante (composition chimique)</term>
<term>Solubilité (MeSH)</term>
<term>Xylème (composition chimique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Phloem</term>
<term>Pinus</term>
<term>Plant Leaves</term>
<term>Plant Roots</term>
<term>Pollen</term>
<term>Populus</term>
<term>Quercus</term>
<term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Feuilles de plante</term>
<term>Phloème</term>
<term>Pinus</term>
<term>Pollen</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Quercus</term>
<term>Racines de plante</term>
<term>Xylème</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Chemical Precipitation</term>
<term>Solubility</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Précipitation chimique</term>
<term>Solubilité</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In this chapter we present a protocol for total protein extraction optimized for wood-forming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17093300</PMID>
<DateCompleted><Year>2007</Year>
<Month>02</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2009</Year>
<Month>11</Month>
<Day>19</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1064-3745</ISSN>
<JournalIssue CitedMedium="Print"><Volume>355</Volume>
<PubDate><Year>2007</Year>
</PubDate>
</JournalIssue>
<Title>Methods in molecular biology (Clifton, N.J.)</Title>
<ISOAbbreviation>Methods Mol Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>Protein extraction from woody plants.</ArticleTitle>
<Pagination><MedlinePgn>37-41</MedlinePgn>
</Pagination>
<Abstract><AbstractText>In this chapter we present a protocol for total protein extraction optimized for wood-forming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Plomion</LastName>
<ForeName>Christophe</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>NRA, UMR BIOGECO, Equipe de Génétique, Cestas, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lalanne</LastName>
<ForeName>Céline</ForeName>
<Initials>C</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Methods Mol Biol</MedlineTA>
<NlmUniqueID>9214969</NlmUniqueID>
<ISSNLinking>1064-3745</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D011232" MajorTopicYN="N">Chemical Precipitation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D052585" MajorTopicYN="N">Phloem</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D028223" MajorTopicYN="N">Pinus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029963" MajorTopicYN="N">Quercus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012995" MajorTopicYN="N">Solubility</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>11</Month>
<Day>10</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2007</Year>
<Month>2</Month>
<Day>3</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
<Month>11</Month>
<Day>10</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">17093300</ArticleId>
<ArticleId IdType="pii">1-59745-227-0:37</ArticleId>
<ArticleId IdType="doi">10.1385/1-59745-227-0:37</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>France</li>
</country>
</list>
<tree><noCountry><name sortKey="Lalanne, Celine" sort="Lalanne, Celine" uniqKey="Lalanne C" first="Céline" last="Lalanne">Céline Lalanne</name>
</noCountry>
<country name="France"><noRegion><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003A89 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003A89 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:17093300
   |texte=   Protein extraction from woody plants.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:17093300" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

Protein extraction from woody plants.

Protein extraction from woody plants.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki